HARS 293T Cell Transient Overexpression Lysate(Denatured)

Catalog # H00003035-T01

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Size:100 uL
Price: USD $ 247.00
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Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
Images
Western Blot
QC Test

Western Blot

Lane 1: HARS transfected lysate ( 57.4 KDa)
Lane 2: Non-transfected lysate.

SDS-PAGE Gel
QC Test

SDS-PAGE Gel

HARS transfected lysate.

  • Specification

    Transfected Cell Line

    293T

    Plasmid

    pCMV-HARS full-length

    Host

    Human

    Theoretical MW (kDa)

    56.1

    Quality Control Testing

    Transient overexpression cell lysate was tested with Anti-HARS antibody (H00003035-B01) by Western Blots.

    Western Blot

    Lane 1: HARS transfected lysate ( 57.4 KDa)
    Lane 2: Non-transfected lysate.

    SDS-PAGE Gel

    HARS transfected lysate.

    Storage Buffer

    1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

    Storage Instruction

    Store at -80°C. Aliquot to avoid repeated freezing and thawing.

  • Applications

    Western Blot

  • Gene Info — HARS

    Entrez GeneID

    3035

    GeneBank Accession#

    NM_002109

    Protein Accession#

    NP_002100

    Gene Name

    HARS

    Gene Alias

    FLJ20491, HRS

    Gene Description

    histidyl-tRNA synthetase

    Omim ID

    142810

    Gene Ontology

    Hyperlink

    Gene Summary

    Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq

    Other Designations

    HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase

  • Interactome
  • Pathway
Contact Info
  • +1-909-264-1399
    +1-909-992-0619
    Toll Free : +1-877-853-6098
  • +1-909-992-3401
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