HARS (Human) IP-WB Antibody Pair
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More Files
- More Functions
Immunoprecipitation of HARS transfected lysate using rabbit polyclonal anti-HARS and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-HARS.
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Specification
Product Description
This IP-WB antibody pair set comes with one antibody for immunoprecipitation and another to detect the precipitated protein in western blot.
Reactivity
Human
Quality Control Testing
Immunoprecipitation-Western Blot (IP-WB)
Immunoprecipitation of HARS transfected lysate using rabbit polyclonal anti-HARS and Protein A Magnetic Bead (U0007), and immunoblotted with mouse polyclonal anti-HARS.
Supplied Product
Antibody pair set content:
1. Antibody pair for IP: rabbit polyclonal anti-HARS (300 ul)
2. Antibody pair for WB: mouse polyclonal anti-HARS (50 ul)
Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
Immunoprecipitation-Western Blot
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Gene Info — HARS
Entrez GeneID
3035Gene Name
HARS
Gene Alias
FLJ20491, HRS
Gene Description
histidyl-tRNA synthetase
Omim ID
142810Gene Ontology
HyperlinkGene Summary
Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins. The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. The gene product is a frequent target of autoantibodies in the human autoimmune disease polymyositis/dermatomyositis. [provided by RefSeq
Other Designations
HisRS|histidine tRNA ligase 1, cytoplasmic|histidine translase|histidine-tRNA ligase
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Interactome
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Pathway
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