GPLD1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GPLD1 transfected lysate.
Western Blot
Lane 1: GPLD1 transfected lysate ( 19.47 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GPLD1 full-length
Host
Human
Theoretical MW (kDa)
19.47
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GPLD1 antibody (H00002822-B01) by Western Blots.
SDS-PAGE Gel
GPLD1 transfected lysate.
Western Blot
Lane 1: GPLD1 transfected lysate ( 19.47 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GPLD1
Entrez GeneID
2822GeneBank Accession#
BC020748.1Protein Accession#
AAH20748.1Gene Name
GPLD1
Gene Alias
GPIPLD, GPIPLDM, MGC22590, PIGPLD, PIGPLD1
Gene Description
glycosylphosphatidylinositol specific phospholipase D1
Omim ID
602515Gene Ontology
HyperlinkGene Summary
Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq
Other Designations
GPI-specific phospholipase D|OTTHUMP00000017721|OTTHUMP00000017722|OTTHUMP00000039407|glycoprotein phospholipase D|glycosylphosphatIdylinositol-specific phospholipase D|glycosylphosphatidylinositol specific phospholipase D1, isoform 2|phospholipase D, pho
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Interactome
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Pathway
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Disease
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