GNAI1 monoclonal antibody (M01), clone 2B8-2A5
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant GNAI1.
Immunogen
GNAI1 (AAH26326, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVAAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYQEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (64.68 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
GNAI1 monoclonal antibody (M01), clone 2B8-2A5. Western Blot analysis of GNAI1 expression in human liver.Western Blot (Transfected lysate)
Western Blot analysis of GNAI1 expression in transfected 293T cell line by GNAI1 monoclonal antibody (M01), clone 2B8-2A5.
Lane 1: GNAI1 transfected lysate(40.4 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to GNAI1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged GNAI1 is approximately 1ng/ml as a capture antibody.ELISA
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Gene Info — GNAI1
Entrez GeneID
2770GeneBank Accession#
BC026326Protein Accession#
AAH26326Gene Name
GNAI1
Gene Alias
Gi
Gene Description
guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 1
Omim ID
139310Gene Ontology
HyperlinkGene Summary
Guanine nucleotide-binding proteins (G proteins) form a large family of signal-transducing molecules. They are found as heterotrimers made up of alpha, beta, and gamma subunits. Members of the G protein family have been characterized most extensively on the basis of the alpha subunit, which binds guanine nucleotide, is capable of hydrolyzing GTP, and interacts with specific receptor and effector molecules. The G protein family includes Gs (MIM 139320) and Gi, the stimulatory and inhibitory GTP-binding regulators of adenylate cyclase; Go, a protein abundant in brain (GNAO1; MIM 139311); and transducin-1 (GNAT1; MIM 139330) and transducin-2 (GNAT2; MIM 139340), proteins involved in phototransduction in retinal rods and cones, respectively (Sullivan et al., 1986 [PubMed 3092218]; Bray et al., 1987 [PubMed 3110783]). Suki et al. (1987) [PubMed 2440724] concluded that the human genome contains at least 3 nonallelic genes for alpha-i-type subunits of G protein; see, e.g, GNAI2 (MIM 139360), GNAI3 (MIM 139370), and GNAIH (MIM 139180).[supplied by OMIM
Other Designations
Gi1 protein alpha subunit
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Interactome
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Publication Reference
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GI protein phosphorylation as marker for scoliosis progression, methods of increasing gipcr signaling subjects.
Alain Moreau, Marie-Yvonne Akoume Ndong.
United States Patent Application Publication 2016 May; [Epub].
Application:WB-Tr, WB-Ti, S-ELISA, ELISA, WB-Re, IHC-P, Human, PBMCs, Myoblasts.
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GI protein phosphorylation as marker for scoliosis progression, methods of increasing gipcr signaling subjects.
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