DGKA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Western Blot
Lane 1: DGKA transfected lysate ( 82.6 KDa)
Lane 2: Non-transfected lysate.
SDS-PAGE Gel
DGKA transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DGKA full-length
Host
Human
Theoretical MW (kDa)
80.96
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DGKA antibody (H00001606-B01) by Western Blots.
Western Blot
Lane 1: DGKA transfected lysate ( 82.6 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
DGKA transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DGKA
Entrez GeneID
1606GeneBank Accession#
NM_001345Protein Accession#
NP_001336Gene Name
DGKA
Gene Alias
DAGK, DAGK1, DGK-alpha, MGC12821, MGC42356
Gene Description
diacylglycerol kinase, alpha 80kDa
Omim ID
125855Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Alternative splicing occurs at this locus and four transcript variants encoding the same protein have been identified. [provided by RefSeq
Other Designations
diacylglycerol kinase alpha|diacylglycerol kinase, alpha (80kD)
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Interactome
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Pathway
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