CYP26A1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CYP26A1 transfected lysate.
Western Blot
Lane 1: CYP26A1 transfected lysate ( 48.6 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CYP26A1 full-length
Host
Human
Theoretical MW (kDa)
48.6
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CYP26A1 antibody (H00001592-B01) by Western Blots.
SDS-PAGE Gel
CYP26A1 transfected lysate.
Western Blot
Lane 1: CYP26A1 transfected lysate ( 48.6 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CYP26A1
Entrez GeneID
1592GeneBank Accession#
NM_057157.1Protein Accession#
NP_476498.1Gene Name
CYP26A1
Gene Alias
CP26, CYP26, P450RAI, P450RAI1
Gene Description
cytochrome P450, family 26, subfamily A, polypeptide 1
Omim ID
602239Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein acts on retinoids, including all-trans-retinoic acid (RA), with both 4-hydroxylation and 18-hydroxylation activities. This enzyme regulates the cellular level of retinoic acid which is involved in regulation of gene expression in both embryonic and adult tissues. Two alternatively spliced transcript variants of this gene, which encode the distinct isoforms, have been reported. [provided by RefSeq
Other Designations
OTTHUMP00000020102|OTTHUMP00000020103|P450, retinoic acid-inactivating, 1|cytochrome P450, subfamily XXVIA, polypeptide 1|retinoic acid 4-hydroxylase|retinoic acid-metabolizing cytochrome
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Interactome
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Pathway
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Disease
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Publication Reference
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The Expression and Prognostic Significance of Retinoic Acid Metabolising Enzymes in Colorectal Cancer.
Brown GT, Cash BG, Blihoghe D, Johansson P, Alnabulsi A, Murray GI.
PLoS One 2014 Mar; 9(3):e90776.
Application：WB-Ce, Human, Embryonic kidney cells.
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The Expression and Prognostic Significance of Retinoic Acid Metabolising Enzymes in Colorectal Cancer.
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