CPT1A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CPT1A full-length
Host
Human
Theoretical MW (kDa)
85.14
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CPT1A antibody (H00001374-B01) by Western Blots.
SDS-PAGE Gel
CPT1A transfected lysate.
Western Blot
Lane 1: CPT1A transfected lysate ( 85.14 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CPT1A
Entrez GeneID
1374GeneBank Accession#
NM_001876.2Protein Accession#
NP_001867.2Gene Name
CPT1A
Gene Alias
CPT1, CPT1-L, L-CPT1
Gene Description
carnitine palmitoyltransferase 1A (liver)
Gene Ontology
HyperlinkGene Summary
The mitochondrial oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase I (which is located in the outer membrane and is detergent-labile) and carnitine palmitoyltransferase II (which is located in the inner membrane and is detergent-stable), together with a carnitine-acylcarnitine translocase. CPT I is the key enzyme in the carnitine-dependent transport across the mitochondrial inner membrane and its deficiency results in a decreased rate of fatty acid beta-oxidation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations
carnitine palmitoyltransferase 1A|carnitine palmitoyltransferase I, liver
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Interactome
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