CIDEA 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
- More Functions
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CIDEA full-length
Host
Human
Theoretical MW (kDa)
27.94
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CIDEA antibody (H00001149-B01) by Western Blots.
Western Blot
Lane 1: CIDEA transfected lysate ( 28.3 KDa)
Lane 2: Non-transfected lysate.SDS-PAGE Gel
CIDEA transfected lysate.
Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CIDEA
Entrez GeneID
1149GeneBank Accession#
NM_198289Protein Accession#
NP_938031Gene Name
CIDEA
Gene Alias
CIDE-A
Gene Description
cell death-inducing DFFA-like effector a
Omim ID
604440Gene Ontology
HyperlinkGene Summary
This gene encodes the homolog of the mouse protein Cidea that has been shown to activate apoptosis. This activation of apoptosis is inhibited by the DNA fragmentation factor DFF45 but not by caspase inhibitors. Mice that lack functional Cidea have higher metabolic rates, higher lipolysis in brown adipose tissue and higher core body temperatures when subjected to cold. These mice are also resistant to diet-induced obesity and diabetes. This suggests that in mice this gene product plays a role in thermogenesis and lipolysis. Two alternative transcripts encoding different isoforms have been identified. [provided by RefSeq
Other Designations
cell death activator
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Interactome
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Disease
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