CHKB 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CHKB transfected lysate.
Western Blot
Lane 1: CHKB transfected lysate ( 43.56 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CHKB full-length
Host
Human
Theoretical MW (kDa)
43.56
Interspecies Antigen Sequence
Mouse (86); Rat (85)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CHKB antibody (H00001120-B01) by Western Blots.
SDS-PAGE Gel
CHKB transfected lysate.
Western Blot
Lane 1: CHKB transfected lysate ( 43.56 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CHKB
Entrez GeneID
1120GeneBank Accession#
NM_005198.3Protein Accession#
NP_005189.2Gene Name
CHKB
Gene Alias
CHETK, CHKL, CKEKB, EKB
Gene Description
choline kinase beta
Gene Ontology
HyperlinkGene Summary
Choline kinase (CK) and ethanolamine kinase (EK) catalyze the phosphorylation of choline/ethanolamine to phosphocholine/phosphoethanolamine. This is the first enzyme in the biosynthesis of phosphatidylcholine/phosphatidylethanolamine in all animal cells. The highly purified CKs from mammalian sources and their recombinant gene products have been shown to have EK activity also, indicating that both activities reside on the same protein. The choline kinase-like protein encoded by CHKL belongs to the choline/ethanolamine kinase family; however, its exact function is not known. Read-through transcripts are expressed from this locus that include exons from the downstream CPT1B locus. [provided by RefSeq
Other Designations
choline kinase-like|choline/ethanolamine kinase
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Interactome
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Pathway
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Disease
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