BLMH 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BLMH transfected lysate.
Western Blot
Lane 1: BLMH transfected lysate ( 50.16 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BLMH full-length
Host
Human
Theoretical MW (kDa)
50.16
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BLMH antibody (H00000642-B01) by Western Blots.
SDS-PAGE Gel
BLMH transfected lysate.
Western Blot
Lane 1: BLMH transfected lysate ( 50.16 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BLMH
Entrez GeneID
642GeneBank Accession#
NM_000386.2Protein Accession#
NP_000377.1Gene Name
BLMH
Gene Alias
BH, BMH
Gene Description
bleomycin hydrolase
Gene Ontology
HyperlinkGene Summary
Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq
Other Designations
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Interactome
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Disease
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