BAG1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BAG1 transfected lysate
Western Blot
Lane 1: BAG1 transfected lysate ( 31.1 KDa).
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BAG1 full-length
Host
Human
Theoretical MW (kDa)
31.1
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BAG1 antibody (H00000573-B01) by Western Blots.
SDS-PAGE Gel
BAG1 transfected lysate
Western Blot
Lane 1: BAG1 transfected lysate ( 31.1 KDa).
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BAG1
Entrez GeneID
573GeneBank Accession#
ENST00000379704Protein Accession#
ENSP00000369026Gene Name
BAG1
Gene Alias
RAP46
Gene Description
BCL2-associated athanogene
Omim ID
601497Gene Ontology
HyperlinkGene Summary
The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites. [provided by RefSeq
Other Designations
BCL2-associated athanogene 1
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Interactome
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Disease
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Publication Reference
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Bcl-2 associated anthanogen-1 (Bag-1) expression and prognostic value in pancreatic head and periampullary cancer.
van der Zee JA, Ten Hagen TL, Hop WC, van Dekken H, Dicheva BM, Seynhaeve AL, Koning GA, Eggermont AM, van Eijck CH.
European Journal of Cancer 2013 Jan; 49(2):323.
Application：WB-Tr, Human , Bag-1 antibody.
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Bcl-2 associated anthanogen-1 (Bag-1) expression and prognostic value in pancreatic head and periampullary cancer.
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