ART4 monoclonal antibody (M01), clone 2F8
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of ART4 expression in transfected 293T cell line by ART4 monoclonal antibody (M01), clone 2F8.
Lane 1: ART4 transfected lysate (Predicted MW: 35.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ART4 is 0.3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ART4.
Immunogen
ART4 (NP_066549, 61 a.a. ~ 160 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SFDDQYQGCSKQVMEKLTQGDYFTKDIEAQKNYFRMWQKAHLAWLNQGKVLPQNMTTTHAVAILFYTLNSNVHSDFTRAMASVARTPQQYERSFHFKYLH
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of ART4 expression in transfected 293T cell line by ART4 monoclonal antibody (M01), clone 2F8.
Lane 1: ART4 transfected lysate (Predicted MW: 35.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ART4 is 0.3 ng/ml as a capture antibody.ELISA
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Gene Info — ART4
Entrez GeneID
420GeneBank Accession#
NM_021071Protein Accession#
NP_066549Gene Name
ART4
Gene Alias
CD297, DO, DOK1
Gene Description
ADP-ribosyltransferase 4 (Dombrock blood group)
Omim ID
110600Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that contains a mono-ADP-ribosylation (ART) motif. It is a member of the ADP-ribosyltransferase gene family but enzymatic activity has not been demonstrated experimentally. Antigens of the Dombrock blood group system are located on the gene product, which is glycosylphosphatidylinosotol-anchored to the erythrocyte membrane. Allelic variants, some of which lead to adverse transfusion reactions, are known. [provided by RefSeq
Other Designations
ADP-ribosyltransferase 4|ADP-ribosyltransferase 4 (DO blood group)|Dombrock blood group carrier molecule|mono-ADP-ribosyltransferase 4
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