AKT1 & FRAP1 Protein Protein Interaction Antibody Pair
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Specifications
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the AKT1 protein, and the other against the FRAP1 protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between AKT1 and FRAP1. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal antibody 1:1200 and anti-FRAP1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. AKT1 rabbit purified polyclonal antibody (100 ug)
2. FRAP1 mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — AKT1
Entrez GeneID
207Gene Name
AKT1
Gene Alias
AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA
Gene Description
v-akt murine thymoma viral oncogene homolog 1
Gene Ontology
HyperlinkGene Summary
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq
Other Designations
RAC-alpha serine/threonine-protein kinase|murine thymoma viral (v-akt) oncogene homolog-1|protein kinase B|rac protein kinase alpha
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Gene Info — MTOR
Entrez GeneID
2475Gene Name
MTOR
Gene Alias
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Gene Description
mechanistic target of rapamycin
Omim ID
601231Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq
Other Designations
FK506 binding protein 12-rapamycin associated protein 1|FK506 binding protein 12-rapamycin associated protein 2|FK506-binding protein 12-rapamycin complex-associated protein 1|FKBP-rapamycin associated protein|FKBP12-rapamycin complex-associated protein 1
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