CAMK2G & FLNA Protein Protein Interaction Antibody Pair
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Representative image of Proximity Ligation Assay of protein-protein interactions between CAMK2G and FLNA. HeLa cells were stained with anti-CAMK2G rabbit purified polyclonal antibody 1:1200 and anti-FLNA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
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Specification
Product Description
This protein protein interaction antibody pair set comes with two antibodies to detect the protein-protein interaction, one against the CAMK2G protein, and the other against the FLNA protein for use in in situ Proximity Ligation Assay. See Publication Reference below.
Reactivity
Human
Quality Control Testing
Protein protein interaction immunofluorescence result.
Representative image of Proximity Ligation Assay of protein-protein interactions between CAMK2G and FLNA. HeLa cells were stained with anti-CAMK2G rabbit purified polyclonal antibody 1:1200 and anti-FLNA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex. The images were analyzed using an optimized freeware (BlobFinder) download from The Centre for Image Analysis at Uppsala University.
Supplied Product
Antibody pair set content:
1. CAMK2G rabbit purified polyclonal antibody (100 ug)
2. FLNA mouse monoclonal antibody (40 ug)
*Reagents are sufficient for at least 30-50 assays using recommended protocols.Storage Instruction
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
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Applications
In situ Proximity Ligation Assay (Cell)
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Gene Info — CAMK2G
Entrez GeneID
818Gene Name
CAMK2G
Gene Alias
CAMK, CAMK-II, CAMKG, FLJ16043, MGC26678
Gene Description
calcium/calmodulin-dependent protein kinase II gamma
Omim ID
602123Gene Ontology
HyperlinkGene Summary
The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Six alternatively spliced variants that encode six different isoforms have been characterized to date. Additional alternative splice variants that encode different isoforms have been described, but their full-length nature has not been determined. [provided by RefSeq
Other Designations
CaM kinase II|OTTHUMP00000019843|OTTHUMP00000019844|calcium/calmodulin-dependent protein kinase (CaM kinase) II gamma
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Gene Info — FLNA
Entrez GeneID
2316Gene Name
FLNA
Gene Alias
ABP-280, ABPX, DKFZp434P031, FLN, FLN1, FMD, MNS, NHBP, OPD, OPD1, OPD2
Gene Description
filamin A, alpha (actin binding protein 280)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an actin-binding protein that crosslinks actin filaments and links actin filaments to membrane glycoproteins. The encoded protein is involved in remodeling the cytoskeleton to effect changes in cell shape and migration. This protein interacts with integrins, transmembrane receptor complexes, and second messengers. Defects in this gene are a cause of several syndromes, including periventricular nodular heterotopias (PVNH1, PVNH4), otopalatodigital syndromes (OPD1, OPD2), frontometaphyseal dysplasia (FMD), Melnick-Needles syndrome (MNS), and X-linked congenital idiopathic intestinal pseudoobstruction (CIIPX). Two transcript variants encoding different isoforms have been found for this gene
Other Designations
OTTHUMP00000024320|actin-binding protein 280|filamin 1|filamin A, alpha
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Interactome
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Disease
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