Products

Product Description

MYCN CISH Probe is designed for the qualitative detection human MYCN gene in formalin-fixed, paraffin-embedded tissue or cells by chromogenic in situ hybridization (CISH).

Reactivity

Human

Recommend Usage

The product is ready-to-use. No reconstitution, mixing, or dilution is required. Bring probe to room temperature (18-25°C) and mix briefly before use.

Supplied Product

Reagent Provided:

Digoxigenin-labeled oligonucleotides targeting sequences mapping in 2p24.3* (chr2:15,846,046-16,213,717) harboring the MYCN gene.
2. Formamide based hybridization buffer.
*According to Human Genome Assembly GRCh37/hg19

Probe Position

Regulatory Status

For research use only (RUO)

Storage Instruction

Store at 2-8°C in an upright position. Return to storage conditions immediately after use.

Note

The probe is intended to be used in combination with the CISH Implementation Kit 2 (Catalog #: KA5366), which provides necessary reagents for specimen pretreatment and post-hybridization processing.

Hybridization signals of digoxigenin-labeled polynucleotides appear as brown- to dark brown colored distinct dots (MYCN gene).
Normal situation: In interphases of normal cells or cells without an amplification involving the MYCN gene locus, two distinct dot-shaped brown signals per nucleus appear.
Aberrant situation: In a cell with amplification of the MYCN gene locus or aneuploidy of chromosome 2, multiple copies of the brown signal or brown signal clusters will be observed.

Interpretation of Result