Abnova offers high quality, efficient and cost-saving conjugation service between antibody/protein and fluorescent dyes, phycobiliproteins (RPE, APC, cl-APC), FITC, streptavidin, biotin and horseradish peroxidase (HRP). Since every antibody/protein has its own unique characteristics, conjugation needs to be optimized. 0.2mg of total antibody/protein of interest will be tested and HPLC analyzed prior to mass labeling. The tested sample will be mixed with mass conjugate product for final purification. We will complete the conjugation within 2-3 weeks from the time we receive the antibody/protein sample.
General Working Progress of Antibody Conjugation
Evaluation and Discussion of Conjugation Requirement and Service
Completion of the Custom Conjugation Specification (PDF, Excel File)
Confirmation of Receipt of Antibody/Protein Sample
Conjugation Buffer Exchange
Antibody/Protein Concentration and Volume Analysis
Test-Scale Conjugation Optimization
Mass Antibody/Protein Labeling
Chromatographic Gel Purification
Spectrophotometer Measurement
COA, Packing, and Shipment
Table of Labeling Molecules and Excitation/Emission Wavelengths
Labeling Molecule
Excitation Max (nm)
Emission Max (nm)
RPE
495, 565
575
APC
650
660
cl-APC (PEAK)
495, 566
660
FITC
495
520
Special Notes:
The antibody/protein of interest should not contain albumin, gelatin, or other amine-containing stabilizers. Purification of antibody/protein from ascites fluid, serum, or cell culture medium or removal of stabilizer proteins prior to conjugation will result in additional cost to the customer.
Minimum quanity of antibody/protein conjugation is 0.1mg. Minimum concentration is 0.2mg/ml.
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