EIF2C2 monoclonal antibody (M01), clone 2E12-1C9   

• Publication Reference

• 1.
• Additional expression of microRNA-181 inhibits Porcine Reproductive and Respiratory Syndrome Virus replication and its implications for controlling virus infection.
  Guo XK, Zhang Q, Gao L, Li N, Chen XX, Feng WH.J Virol. 2012 Nov 14.

• 2.
• Direct Conversion of Fibroblasts to Neurons by Reprogramming PTB-Regulated MicroRNA Circuits.
  Xue Y, Ouyang K, Huang J, Zhou Y, Ouyang H, Li H, Wang G, Wu Q, Wei C, Bi Y, Jiang L, Cai Z, Sun H, Zhang K, Zhang Y, Chen J, Fu XD.Cell. 2013 Jan 9. pii: S0092-8674(12)01433-X. doi: 10.1016/j.cell.2012.11.045.

• 3.
• siRNA Has Greatly Elevated Mismatch Tolerance at 3'-UTR Sites.
  Wei N, Zhang L, Huang H, Chen Y, Zheng J, Zhou X, Yi F, Du Q, Liang Z.PLoS One. 2012;7(11):e49309. doi: 10.1371/journal.pone.0049309. Epub 2012 Nov 8.

• 4.
• The mammalian TRIM-NHL protein TRIM71/LIN-41 is a repressor of mRNA function.
  Loedige I, Gaidatzis D, Sack R, Meister G, Filipowicz W.Nucleic Acids Res. 2012 Nov 3. [Epub ahead of print]

• 5.
• MicroRNA-155 promotes atherosclerosis by repressing Bcl6 in macrophages.
  Nazari-Jahantigh M, Wei Y, Noels H, Akhtar S, Zhou Z, Koenen RR, Heyll K, Gremse F, Kiessling F, Grommes J, Weber C, Schober A.J Clin Invest. 2012 Nov 1;122(11):4190-202. doi: 10.1172/JCI61716. Epub 2012 Oct 8.

• 6.
• The p38/MK2-Driven Exchange between Tristetraprolin and HuR Regulates AU-Rich Element-Dependent Translation.
  Tiedje C, Ronkina N, Tehrani M, Dhamija S, Laass K, Holtmann H, Kotlyarov A, Gaestel M.PLoS Genet. 2012 Sep;8(9):e1002977. doi: 10.1371/journal.pgen.1002977. Epub 2012 Sep 27.

• 7.
• HIV-1 Gag co-opts a cellular complex containing DDX6, a helicase that facilitates capsid assembly.
  Reed JC, Molter B, Geary CD, McNevin J, McElrath J, Giri S, Klein KC, Lingappa JR.J Cell Biol. 2012 Aug 6;198(3):439-56. Epub 2012 Jul 30.

• 8.
• Generation of miRNA sponge constructs.
  Kluiver J, Slezak-Prochazka I, Smigielska-Czepiel K, Halsema N, Kroesen BJ, van den Berg A.Methods. 2012 Jul 23.

• 9.
• MicroRNAs Regulate p21(Waf1/cip1) Protein Expression and the DNA Damage Response in Human Embryonic Stem Cells.
  Dolezalova D, Mraz M, Barta T, Plevova K, Vinarsky V, Holubcova Z, Jaros J, Dvorak P, Pospisilova S, Hampl A.Stem Cells. 2012 Apr 17. doi: 10.1002/stem.1108.

• 10.
• A Pathogenic Mechanism in Huntington's Disease Involves Small CAG-Repeated RNAs with Neurotoxic Activity.
  Banez-Coronel M, Porta S, Kagerbauer B, Mateu-Huertas E, Pantano L, Ferrer I, Guzman M, Estivill X, Marti E.PLoS Genet. 2012 Feb;8(2):e1002481. Epub 2012 Feb 23.

• 11.
• Regulation of Cyclin T1 and HIV-1 Replication by MicroRNAs in Resting CD4+ T Lymphocytes.
  Chiang K, Sung TL, Rice AP.J Virol. 2012 Mar;86(6):3244-52. Epub 2011 Dec 28.

• 12.
• Regulation of human chondrocyte function through direct inhibition of cartilage master-regulator SOX9 by miRNA-145.
  Martinez-Sanchez A, Dudek KA, Murphy CL.J Biol Chem. 2011 Nov 18.

• 13.
• Fragile X Related Protein 1 Clusters with Ribosomes and Messenger RNAs at a Subset of Dendritic Spines in the Mouse Hippocampus.
  Cook D, Del Rayo Sanchez-Carbente M, Lachance C, Radzioch D, Tremblay S, Khandjian EW, Desgroseillers L, Murai KK.PLoS One. 2011;6(10):e26120. Epub 2011 Oct 11.

• 14.
• Characterization of extracellular circulating microRNA.
  Turchinovich A, Weiz L, Langheinz A, Burwinkel B.Nucleic Acids Res. 2011 May 24. [Epub ahead of print]

• 15.
• Limiting Ago protein restricts RNAi and microRNA biogenesis during early development in Xenopus laevis.
  Lund E, Sheets MD, Imboden SB, Dahlberg JE.Genes Dev. 2011 Jun 1;25(11):1121-31. Epub 2011 May 16.

• 16.
• HuR-dependent loading of miRNA RISC to the mRNA encoding the Ras-related small GTPase RhoB controls its translation during UV-induced apoptosis.
  Glorian V, Maillot G, Poles S, Iacovoni JS, Favre G, Vagner S.Cell Death Differ. 2011 Apr 29. [Epub ahead of print]

• 17.
• Viral RNAi Suppressor Reversibly Binds siRNA to Outcompete Dicer and RISC via Multiple Turnover.
  Rawlings RA, Krishnan V, Walter NG.J Mol Biol. 2011 Feb 24. [Epub ahead of print]

• 18.
• The QKI-6 RNA Binding Protein Localizes with the MBP mRNAs in Stress Granules of Glial Cells.
  Wang Y, Lacroix G, Haines J, Doukhanine E, Almazan G, Richard S.PLoS One. 2010 Sep 17;5(9). pii: e12824.

• 19.
• A dicer-independent miRNA biogenesis pathway that requires Ago catalysis.
  Cheloufi S, Dos Santos CO, Chong MM, Hannon GJ.Nature. 2010 Jun 3;465(7298):584-9.

• 20.
• P-Body Loss Is Concomitant with Formation of a Messenger RNA Storage Domain in Mouse Oocytes.
  Flemr M, Ma J, Schultz RM, Svoboda P.Biol Reprod. 2010 May;82(5):1008-17. Epub 2010 Jan 14.

• 21.
• RNA binding motif protein 4 translocates to cytoplasmic granules and suppresses translation via Argonaute2 during muscle cell differentiation.
  Lin JC, Tarn WY.J Biol Chem. 2009 Dec 11;284(50):34658-65. Epub 2009 Sep 29.

• 22.
• A high throughput experimental approach to identify miRNA targets in human cells.
  Tan LP, Seinen E, Duns G, de Jong D, Sibon OC, Poppema S, Kroesen BJ, Kok K, van den Berg A.Nucleic Acids Res. 2009 Sep 4. [Epub ahead of print]

• 23.
• Existence of a microRNA pathway in anucleate platelets.
  Landry P, Plante I, Ouellet DL, Perron MP, Rousseau G, Provost P.Nat Struct Mol Biol. 2009 Sep;16(9):961-6. Epub 2009 Aug 9.

• 24.
• Dynamic Interaction between P-Bodies and Transport Ribonucleoprotein Particles in Dendrites of Mature Hippocampal Neurons.
  Zeitelhofer M, Karra D, Macchi P, Tolino M, Thomas S, Schwarz M, Kiebler M, Dahm R.J Neurosci. 2008 Jul 23;28(30):7555-62.

• 25.
• Argonaute2 Is Essential for Mammalian Gastrulation and Proper Mesoderm Formation.
  Alisch RS, Jin P, Epstein M, Caspary T, Warren ST.PLoS Genet. 2007 Dec 28;3(12):e227.

• 26.
• Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells.
  Vickers TA, Lima WF, Nichols JG, Crooke ST.Nucleic Acids Res. 2007;35(19):6598-610. Epub 2007 Sep 28.

• 27.
• Ribonuclease Dicer Cleaves Triplet Repeat Hairpins into Shorter Repeats that Silence Specific Targets.
  Krol J, Fiszer A, Mykowska A, Sobczak K, de Mezer M, Krzyzosiak WJ.Mol Cell. 2007 Feb 23;25(4):575-86.

• Applications

• Western Blot (Recombinant protein)
• Protocol Download

• Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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• Immunoperoxidase of monoclonal antibody to EIF2C2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml]
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• Immunofluorescence
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• Immunofluorescence of monoclonal antibody to EIF2C2 on HeLa cell. [antibody concentration 10 ug/ml]
• Protocol Download

• Sandwich ELISA (Recombinant protein)
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• Detection limit for recombinant GST tagged EIF2C2 is 0.3 ng/ml as a capture antibody.
• Protocol Download

• ELISA

• RNAi Knockdown (Antibody validated)
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• Western blot analysis of EIF2C2 over-expressed 293 cell line, cotransfected with EIF2C2 Validated Chimera RNAi ( Cat # H00027161-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2C2 monoclonal antibody (M01), clone 2E12-1C9 (Cat # H00027161-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
• Protocol Download