Rabbit polyclonal antibody raised against synthetic peptide of HDAC1.
Immunogen:
A synthetic peptide corresponding to C-terminus of human HDAC1.
Host:
Rabbit
Theoretical MW (kDa):
62
Reactivity:
Human, Mouse, Rat
Specificity:
This antibody detects the 62 KDa histone deacetylase 1. It does not cross-react with other HDAC proteins including HDAC2, 3, 4, 5, 6, 7, and 8.
Form:
Liquid
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
Western Blot (0.5-4 ug/mL) Immunoprecipitation (10-20 ug/mL) Immunohistochemistry (10-20 ug/mL) The optimal working dilution should be determined by the end user.
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains thimerosal: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot
Western blot analysis of HDAC1 expression in lysates from mouse small intestine (Lane 1) , Jurkat cells (Lane 2) , and rat kidney (Lane 3) . The antibody detects the 62 kDa HDAC1.
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq
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