Rabbit polyclonal antibody raised against synthetic peptide of SOX2.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human SOX2.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Purification:
Ammonium sulfate precipitation
Recommend Usage:
ELISA (1:1000) Western Blot (1:50-100) Immunohistochemistry (1:10-50) Immunofluorescence (1:10-50) Flow cytometry (1:10-50) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of SOX2 polyclonal antibody (Cat # PAB3876) in 293 cell line lysates (35 ug/lane). SOX2 (arrow) was detected using the purified polyclonal antibody.
Western Blot (Transfected lysate)
Western blot analysis of SOX2 (arrow) using rabbit SOX2 polyclonal antibody (Cat # PAB3876). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SOX2 gene (Lane 2) (Origene Technologies).
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with SOX2 polyclonal antibody (Cat # PAB3876) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence
Immunofluorescence analysis of SOX2 polyclonal antibody (Cat # PAB3876) in HeLa cells. 0.025 mg/mL primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.
ELISA
Flow Cytometry
Flow cytometric analysis of NCI-H292 cells using SOX2 polyclonal antibody (Cat # PAB3876)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq
Other Designations:
SRY-related HMG-box gene 2,sex-determining region Y-box 2,transcription factor SOX2
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