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BUB1B polyclonal antibody   

  • Katalog # : PAB3263
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  • 100 ug
  • EUR € 289
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against synthetic peptide of BUB1B.
  • Immunogen:
  • A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human BUB1B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Purification:
  • Protein G purification
  • Recommend Usage:
  • ELISA (1:1000)
    Western Blot (1:50-200)
    Immunohistochemistry (1:50-100)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In PBS (0.09% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western blot analysis of BUB1B (arrow) using rabbit BUB1B polyclonal antibody (Cat # PAB3263). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BUB1B polyclonal antibody (Cat # PAB3263) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
  • ELISA
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 701
  • Gene Name:
  • BUB1B
  • Gene Alias:
  • BUB1beta,BUBR1,Bub1A,MAD3L,SSK1,hBUBR1
  • Gene Description:
  • budding uninhibited by benzimidazoles 1 homolog beta (yeast)
  • Gene Summary:
  • This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq
  • Other Designations:
  • BUB1 budding uninhibited by benzimidazoles 1 homolog beta,MAD3/BUB1-related protein kinase,budding uninhibited by benzimidazoles 1, beta
  • Interactome
  • Gene Pathway
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