Goat polyclonal antibody raised against synthetic peptide of AKT2.
Immunogen:
A synthetic peptide corresponding to C-terminus of human AKT2.
Sequence:
C-RYDSLGLLELDQRT
Host:
Goat
Theoretical MW (kDa):
60
Reactivity:
Human
Form:
Liquid
Purification:
Antigen affinity purification
Recommend Usage:
ELISA (1:16000) Western Blot (0.1-0.3 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Tissue lysate)
AKT2 polyclonal antibody (Cat # PAB24587) (0.3 ug/mL) staining of human skeletal muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
This gene is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains. The gene was shown to be amplified and overexpressed in 2 of 8 ovarian carcinoma cell lines and 2 of 15 primary ovarian tumors. Overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phophorylating several known proteins. [provided by RefSeq
Other Designations:
Murine thymoma viral (v-akt) homolog-2,rac protein kinase beta