Rabbit polyclonal antibody raised against synthetic peptide of BMPR2.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to N-terminus of human BMPR2.
Host:
Rabbit
Reactivity:
Human, Mouse
Form:
Liquid
Purification:
Protein G purification
Recommend Usage:
ELISA (1:1000) Western Blot (1:100-500) Immunohistochemistry (1:50-100) Flow cytometry (1:10-50) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.09% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot analysis of BMPR2 polyclonal antibody (Cat # PAB1907) in mouse heart tissue lysate. BMPR2 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Formalin-fixed and paraffin-embedded human hepatocarcinoma (A) and human kidney (B) tissue reacted with BMPR2 polyclonal antibody (Cat # PAB1907), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Immunofluorescence
Immunofluorescence analysis of BMPR2 polyclonal antibody (Cat # PAB1907) in frozen section of human brain tissue. 0.005 mg/mL primary antibody was followed by FITC-conjugated anti-rabbit antibody. FITC-conjugated anti-rabbit antibody emits green fluorescence. Blue counterstaining is Hoechst 33258. This image kindly offered by Chunqing Zhang, Third Military Medical University.
ELISA
Flow Cytometry
Flow cytometric analysis of HepG2 cells using BMPR2 polyclonal antibody (Cat # PAB1907)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
This gene encodes a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of two different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension, both familial and fenfluramine-associated, and with pulmonary venoocclusive disease. [provided by RefSeq
Other Designations:
BMP type II receptor,bone morphogenetic protein receptor type II,bone morphogenetic protein receptor type-2,primary pulmonary hypertension 1,serine/threonine kinase,type II activin receptor-like kinase,type II receptor for bone morphogenetic protein-4
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