Goat polyclonal antibody raised against synthetic peptide of MYB.
Immunogen:
A synthetic peptide corresponding to amino acids 557-569 at internal region of human MYB.
Sequence:
C-HRDQTVKTQKENT
Host:
Goat
Theoretical MW (kDa):
85
Reactivity:
Human
Specificity:
This antibody is expected to recognize all reported isofoms. (NP_001123645.1; NP_005366.2; NP_001123644.1; NP_001155128.1; NP_001155129.1; NP_001155130.1; NP_001155131.1; NP_001155132.1).
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:8000) Western Blot (0.5-1.5 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
MYB polyclonal antibody (Cat # PAB18570) (0.5 ug/mL) staining of Jurkat lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
This gene encodes a transcription factor that is a member of the MYB family of transcription factor genes. The protein contains three domains, an N-terminal DNA-binding domain, a central transcriptional activation domain and a C-terminal domain involved in transcriptional repression. This protein plays an essential role in the regulation of hematopoiesis and may play a role in tumorigenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq