Rabbit polyclonal antibody raised against full length recombinant CSF2.
Immunogen:
Recombinant protein corresponding to full length human CSF2.
Host:
Rabbit
Reactivity:
Human
Form:
Lyophilized
Recommend Usage:
ELISA (1:1000-1:5000) Western Blot (1:500-1:2000) The optimal working dilution should be determined by the end user.
Storage Buffer:
Lyophilized from 20 mM KH2PO4, 150 mM NaCl, pH 7.2
Storage Instruction:
Store at 4°C on dry atmosphere. After reconstitution with 0.1 mL of deionized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western blot using Csf2 polyclonal antibody (Cat # PAB16920) shows detection of a band ~15 KDa in size corresponding to recombinant human Csf2. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG secondary antibody diluted 1 : 20,000 in blocking buffer. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. [provided by RefSeq