Rabbit polyclonal antibody raised against recombinant HDAC1.
Immunogen:
Recombinant protein corresponding to human HDAC1.
Host:
Rabbit
Reactivity:
Human
Form:
Liquid
Recommend Usage:
Western Blot (1:1000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In serum (0.05% sodium azide)
Storage Instruction:
Store at -20°C. For long term storage store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot was performed using nuclear extracts from HeLa cells (HeLa NE, 20 ug) and the HDAC1 polyclonal antibody (Cat # PAB14045) at dilution 1 : 1,000.
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq