Rabbit polyclonal antibody raised against synthetic phosphopeptide of AKT1.
Synthetic phosphopeptide corresponding to residues surrounding S473 of human AKT1.
Dog, Human, Mouse, Rat
The motif corresponding to the major autophosphorylation site of the human AKT1. This antibody does not cross react to the non-phosphorylated AKT1 or with other unrelated phosphorylated serine. Ser473 corresponds to QFSYS in human, rat, mouse, chicken and canis of AKT/PKB proteins.
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Western Blot (0.1-0.2 ug/mL) ELISA (0.01-0.1 ug/mL) Immunoprecipitation (2-5 ug/mL) Immunohistochemistry (1-2 ug/mL) The optimal working dilution should be determined by the end user.
In TBS, pH 7.2 (10% Proclin300)
Store at 4°C. For long term storage store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Western Blot (Cell lysate)
The whole cell lysate derived from PDGF stimulated NIH/3T3 was immunoprobed by AKT1 (phospho S473) polyclonal antibody (Cat # PAB12600) at 1 : 1000. An immunoreactive band was observed around ~ 60 KDa (A). This band is abolished by pre-incubation with immunizing peptide (B).
Dot Blot (Peptide)
Dot Blot : 1 ug peptide was blot onto NC membrane (A-Phospho-AKT1 S473 ; B-AKT1 (Nonphospho) ; C : Non-related Phosphopeptide) was blotted by AKT1 (phospho S473) polyclonal antibody (Cat # PAB12600) at a 1 : 2000 dilution.
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq