Western blot using CDKN1B polyclonal antibody (Cat # PAB10300) shows detection of CDKN1B protein in MCF-7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1 : 10,000 dilution of Dylight™ 800 conjugated Gt-a-Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).
This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq