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CDKN1B polyclonal antibody   

  • Katalog # : PAB10300
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  • 100 uL
  • EUR € 375
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  • Specification
  • Product Description:
  • Rabbit polyclonal antibody raised against full length recombinant CDKN1B.
  • Immunogen:
  • Recombinant protein corresponding to full length human CDKN1B.
  • Host:
  • Rabbit
  • Reactivity:
  • Human
  • Specificity:
  • Antiserum will specifically react with a p27 protein from human tissue. No reaction was observed against other related tumor suppressor proteins. No reactivity is observed with p27 from mouse.
  • Form:
  • Liquid
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.
  • Recommend Usage:
  • ELISA (1:10000-1:40000)
    Western Blot (1:1000-1:4000)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Publication Reference
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot using CDKN1B polyclonal antibody (Cat # PAB10300) shows detection of CDKN1B protein in MCF-7 whole cell lysate (lanes 1-3) (arrowhead).
    Separation was achieved using a 4-20% gradient gel.
    Blocking occurred using 5% BLOTTO.
    Primary antibody was diluted 1:500 in 1% BLOTTO.
    The membrane was washed and reacted with a 1 : 10,000 dilution of Dylight™ 800 conjugated Gt-a-Rabbit IgG.
    Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (Lane M).
  • Immunoprecipitation
  • ELISA
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunoprecipitation
  • ELISA
  • Gene Information
  • Entrez GeneID:
  • 1027
  • Protein Accession#:
  • P46527;NP_004055
  • Gene Name:
  • CDKN1B
  • Gene Alias:
  • CDKN4,KIP1,MEN1B,MEN4,P27KIP1
  • Gene Description:
  • cyclin-dependent kinase inhibitor 1B (p27, Kip1)
  • Gene Summary:
  • This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. [provided by RefSeq
  • Other Designations:
  • cyclin-dependent kinase inhibitor 1B
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