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- Home > Products > Polyclonal Ab > Human > Apoptosis > HDAC1 polyclonal antibody
HDAC1 polyclonal antibody
- Katalog # : PAB10217
- Visit Frequency :
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- 100 ug
- EUR € 375
- order now, ship in 5 days
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Phone : +49-6221-825877
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- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of HDAC1.
- Immunogen:
- A synthetic peptide corresponding to amino acids 466-482 of human HDAC1.
- Host:
- Rabbit
- Reactivity:
- Bovine, Chicken, Chimpanzee, Human, Mouse, Rat
- Form:
- Liquid
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:10000-1:50000)
Western Blot (1:1000-1:5000)
Immunohistochemistry (1:200-1:1000)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In 20 mM KH2PO4, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain.
Unoki M, Nishidate T, Nakamura Y.Oncogene. 2004 Oct 7;23(46):7601-10.
- 2.
- Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1.
Zhao Q, Cumming H, Cerruti L, Cunningham JM, Jane SM.J Biol Chem. 2004 Oct 1;279(40):41477-86. Epub 2004 Jul 24.
- 3.
- Restoration of transforming growth factor-beta signaling through receptor RI induction by histone deacetylase activity inhibition in breast cancer cells.
Ammanamanchi S, Brattain MG.J Biol Chem. 2004 Jul 30;279(31):32620-5. Epub 2004 May 20.
- Applications
- Western Blot (Cell lysate)
- Western blot using HDAC1 polyclonal antibody (Cat # PAB10217) shows detection of a band at ~65 KDa corresponding to human HDAC1 present in a 293 whole cell lysate (arrowhead).
Approximately 35 ug of lysate wseparated on a 4-20% Tris-HEPES gel by SDS-PAGE and transferred onto nitrocellulose.
After blocking the membrane was probed with the primary antibody diluted to 1:1,350.
Reaction occurred 2 h at room temperature followed by washes and reaction with a 1 : 10,000 dilution of IRDyeas™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) shows strong nuclear staining of tumor cells in human lung tissue.
Tissue was formalin-fixed and paraffin embedded.
Brown color indicates presence of protein, blue color shows cell nuclei.
Personal Communication, Kenneth Wester, www.proteinatlas.org, Upsala, Sweden.
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Immunohistochemistry of HDAC1 polyclonal antibody (Cat # PAB10217) was used at a 1 : 500 dilution to detect HDAC1 by immunohistochemistry in human prostate cancer tissue.
Tissue was formalin-fixed and paraffin embedded.
Personal Communication, Alan Yen, Life Span Biosciences, Seattle, WA.
- Entrez GeneID:
- 3065
- Protein Accession#:
- Q13547;NP_004955
- Gene Name:
- HDAC1
- Gene Alias:
- DKFZp686H12203,GON-10,HD1,RPD3,RPD3L1
- Gene Description:
- histone deacetylase 1
- Omim ID:
- 601241
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq
- Other Designations:
- OTTHUMP00000008745,reduced potassium dependency, yeast homolog-like 1
