The activity is determined by the dose-dependent proliferation of murine BALB/c 3T3 cells. The expected ED50 for this effect is 16-24 pg/mL.
Storage Buffer:
No additive
Storage Instruction:
Store at -20°C on dry atmosphere. After reconstitution with sterilized water, store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note:
Result of activity analysis
3T3 cells were cultured with 0 to 1 ng/mL human EGF. Cell proliferation was measured after 40 hours and the linear portion of the curve was us used to calculate the ED50.
Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. [provided by RefSeq