Western blot analysis of HeLa cell lysate (each 30 ug) were resolved by SDS - PAGE , transferred to PVDF membrane and probed with GSK3B monoclonal antibody , clone 1F7 (1 : 2000) (Cat # MAB1087). Proteins were visualized using a goat anti - mouse secondary antibody conjugated to HRP and an ECL detection system.
The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene