Mouse monoclonal antibody raised against partial recombinant BIRC5.
Recombinant protein corresponding to human BIRC5.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In PBS (0.5% protein stabilizer, 0.05% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Transfected lysate)
Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against HEK293 (1) and BIRC5 (aa 1-142) -hIgGFc transfected HEK293 (2) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using BIRC5 monoclonal antibody, clone 1H5 (Cat # MAB10608) against human BIRC5 (aa 1-142) recombinant protein. (Expected MW is 16 kDa)
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq
apoptosis inhibitor 4,baculoviral IAP repeat-containing protein 5,survivin variant 3 alpha