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BID monoclonal antibody, clone 3C5   

  • Katalog # : MAB10503
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  • 100 uL
  • EUR € 565
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    Phone : +49-6221-825877
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against partial recombinant BID.
  • Immunogen:
  • Recombinant protein corresponding to human BID.
  • Host:
  • Mouse
  • Theoretical MW (kDa):
  • 22
  • Reactivity:
  • Human
  • Form:
  • Liquid
  • Isotype:
  • IgG1
  • Recommend Usage:
  • ELISA (1:10000)
    Western Blot (1:500-1:2000)
    Immunohistochemistry (1:200-1:1000)
    Immunofluorescence (1:200-1:1000)
    Flow cytometry (1:200-1:400)
    The optimal working dilution should be determined by the end user.
  • Storage Buffer:
  • In ascites (0.03% sodium azide)
  • Storage Instruction:
  • Store at 4°C. For long term storage store at -20°C.
    Aliquot to avoid repeated freezing and thawing.
  • Note:
  • This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Applications
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • Western blot analysis using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) against HeLa (1), A-431 (2), Jurkat (3), A-549 (4), HepG2 (5), and HEK293 (6) cell lysate.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemical analysis of paraffin-embedded human prostate tissues (A) and tonsil tissues (B) using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) with DAB staining.
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
  • ELISA
  • ELISA
  • ELISA detection with BID monoclonal antibody, clone 3C5 (Cat # MAB10503).
  • Flow Cytometry
  • Flow Cytometry
  • Flow cytometric analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green) and negative control (purple).
  • Application Image
  • Western Blot (Cell lysate)
  • Western Blot (Cell lysate)
  • enlarge
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 637
  • Gene Name:
  • BID
  • Gene Alias:
  • FP497,MGC15319,MGC42355
  • Gene Description:
  • BH3 interacting domain death agonist
  • Gene Summary:
  • This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
  • Other Designations:
  • BH3-interacting domain death agonist,BID isoform ES(1b),BID isoform L(2),BID isoform Si6,Human BID coding sequence,OTTHUMP00000196197,apoptic death agonist,desmocollin type 4
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