Mouse monoclonal antibody raised against partial recombinant BID.
Recombinant protein corresponding to human BID.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Cell lysate)
Western blot analysis using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) against HeLa (1), A-431 (2), Jurkat (3), A-549 (4), HepG2 (5), and HEK293 (6) cell lysate.
Immunohistochemical analysis of paraffin-embedded human prostate tissues (A) and tonsil tissues (B) using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) with DAB staining.
Immunofluorescence analysis of HeLa cells using BID monoclonal antibody, clone 3C5 (Cat # MAB10503) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
BH3-interacting domain death agonist,BID isoform ES(1b),BID isoform L(2),BID isoform Si6,Human BID coding sequence,OTTHUMP00000196197,apoptic death agonist,desmocollin type 4