Mouse monoclonal antibody raised against synthetic peptide of BCL2.
Immunogen:
A synthetic peptide (conjugated with KLH) corresponding to human BCL2.
Host:
Mouse
Theoretical MW (kDa):
26
Reactivity:
Human, Mouse
Form:
Liquid
Isotype:
IgG1
Recommend Usage:
ELISA (1:10000) Immunohistochemistry (1:200-1:1000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
Storage Buffer:
In ascites (0.03% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Immunohistochemical analysis of paraffin-embedded human colon cancer tissues (A) and human brain tissues (B) using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) with DAB staining.
Immunofluorescence
Immunofluorescence analysis of NIH/3T3 cells using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
ELISA
Flow Cytometry
Flow cytometric analysis of 3T3-L1 cells using BCL2 monoclonal antibody, clone 8E12 (Cat # MAB10499) (green) and negative control (purple).
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq
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