Mouse monoclonal antibody raised against partial recombinant APOB.
Recombinant protein corresponding to human APOB.
Theoretical MW (kDa):
ELISA (1:10000) Western Blot (1:500-1:2000) Immunofluorescence (1:200-1:1000) Flow cytometry (1:200-1:400) The optimal working dilution should be determined by the end user.
In ascites (0.03% sodium azide)
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Western Blot (Recombinant protein)
Western blot analysis using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) against human ApoB (aa : 3900-4110) recombinant protein. (Expected MW is 515.6 kDa)
Immunofluorescence analysis of HepG2 cells using APOB monoclonal antibody, clone 6G6 (Cat # MAB10489) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq