Mouse monoclonal antibody raised against recombinant ASS1.
Immunogen:
Recombinant protein corresponding to human ASS1.
Host:
Mouse
Theoretical MW (kDa):
47
Reactivity:
Human, Monkey, Mouse
Form:
Liquid
Recommend Usage:
Western Blot (1:500-1:2000) Immunohistochemistry (1:200-1:1000) ELISA (1:10000) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS (0.05% sodium azide, 0.5% protein stabilizer)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Cell lysate)
Western blot analysis of ASS1 monoclonal antibody, clone 2C10 (Cat # MAB10449) against A-431 (1), RAJI (2), MOLT4 (3), Jurkat (4), A-549 (5), NIH/3T3 (6), PC-12 (7) and COS-7 (8) cell lysate.
Western Blot (Recombinant protein)
Western blot analysis using ASS1 monoclonal antibody, clone 2C10 (Cat # MAB10449) against human ASS1 (aa 40-236) recombinant protein. (Expected MW is 47 kDa)
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue (A) and colon cancer tissue (B) using ASS1 monoclonal antibody, clone 2C10 (Cat # MAB10449) with DAB staining.
The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
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