A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Theoretical MW (kDa):
Human, Non-Human Primates
CD14, GPI (glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. The antibody also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
Size-exclusion chromatography purification
Flow Cytometry (10 uL reagent/100 uL of whole blood or 106 cells) The optimal working dilution should be determined by the end user.
In PBS (0.2% BSA, 15 mM sodium azide)
Store at 4°C. Do not freeze.
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Surface staining of human peripheral blood cells with CD14 monoclonal antibody, clone MEM-15 (PE) (Cat # MAB5064).
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells. Alternative splicing results in multiple transcript variants encoding the same isoform. [provided by RefSeq