Transient overexpression cell lysate was tested with Anti-CFH antibody (H00003075-D01P) by Western Blots. SDS-PAGE Gel CFH transfected lysate. Western Blot Lane 1: CFH transfected lysate ( 51.00 KDa) Lane 2: Non-transfected lysate.
Storage Buffer:
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
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