Transient overexpression cell lysate was tested with Anti-ECHS1 antibody (H00001892-B01) by Western Blots. SDS-PAGE Gel ECHS1 transfected lysate. Western Blot Lane 1: ECHS1 transfected lysate ( 32.01 KDa) Lane 2: Non-transfected lysate.
Storage Buffer:
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq
Other Designations:
OTTHUMP00000020811,mitochondrial short-chain enoyl-coenzyme A hydratase 1