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Protemist® DT II - 高产量蛋白表示
Abnova推出新一代全自动的桌面机器人蛋白合成器，Protemist® DT II，用于高产量的Eykaryotic蛋白生产。该系统由CellFree科技有限公司开发和制造。它使用一种特殊的小麦胚溶解物和一个双层反应器，用来合成具有重要意义的蛋白。Protemist® DT II可以运行两种规模的转化反应，1.2 ml （24孔）或6 ml （6孔）的反应规模。这是用于转录、转化和提纯的专家级系统。所设计的试验方案和自动化易于操作，避免了手工处理或生产。
Wheat germ cell-free protein synthesis
Start with expression vector and gene of interest
Transcription, translation, and purification in less than 35 hours
Batch affinity purification for 6-well format
Approximately 0.5 mg/well (6-well format) or 0.1 mg/well (24-well format) of crude protein production
Wide temperature control range for purification (4°C to 37°C)
Control, record, and data retrieval by PC
Disposable containers, tips, and tubes
Programs for protein synthesis and purification
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1.2-ml translation scale
6-ml translation scale
6-ml scale translation of GST-tagged protein and its purification using reduced glutathione buffer for elution
6-ml scale translation of GST-tagged protein, and its tag removal and purification in the presence of TEV protease
6-ml scale translation of GST-tagged protein, and its tag removal and purification in the presence of PreScission protease
6-ml scale translation of His-tagged protein and its purification using imidazole buffer for elution
6-ml scale translation of His-tagged protein, and its tag removal and purification in the presence of TEV protease
Demonstrations
GST fusion protein expression and purification
Translation (6-ml format) with WEPRO 1240G at 15°C for 20hrs, purification with glutathione-sepharose followed by elution with 1 ml reduction-type glutathione
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