世界上最大的抗体和重组蛋白制造商
|
首页
|
联系我们
| Language
▼
English
Deutsch
日本語
简体中文
繁體中文
产品
优惠
新产品
新应用
焦点产品浏览
产品地图
服务
综合解决方案及服务
基因合成
PCR组合
多肽與磷酸化
设计和合成
抗原表位定位
蛋白表达
小麦胚蛋白表達
需要小鼠蛋白?
抗体产品生产
小鼠单克隆抗体
小鼠和兔源多克隆抗体
兔源单克隆抗体
产量扩增
腹水
CELLine
®
瞬时转染
稳定细胞系
抗体标记
单克隆抗体
重组蛋白
免疫组化
基因表达谱
抗体配对
FISH 探針
循环肿瘤细胞
伴随诊断
支持
常见问题
在线订购教学
实验指南
操作问题指南
目录和传单
期刊文献
AbVideo™
资源
抗体标记
细胞凋亡
循环肿瘤细胞
ELISA Pairs Kits
Epigenetics
FISH
免疫组化
线粒体
神经科学
次要抗体
干细胞
有用的网页链接
技术
Proximity Ligation Assay配对抗体
小鼠单克隆抗体
兔源单克隆抗体
嵌合RNAi
荧光原位杂交(FISH)
膜蛋白质
Protein+Antibody+Mass
小麦胚蛋白
联系
研究者奖励
抗体学院
试剂商业化
合作
客户证言
电子报
Facebook
关于我们
使命
新闻
eProcurement
100%品质保证
联系我们
Advanced
登陆
我的帐户
感兴趣产品
订单状况
购物车
Member Login
Email:
Password:
Forget your password
支持
常见问题
在线订购教学
实验指南
操作问题指南
目录和传单
期刊文献
AbVideo™
资源
抗体标记
细胞凋亡
循环肿瘤细胞
ELISA Pairs Kits
Epigenetics
FISH
免疫组化
线粒体
神经科学
次要抗体
干细胞
有用的网页链接
技术
Proximity Ligation Assay配对抗体
小鼠单克隆抗体
兔源单克隆抗体
嵌合RNAi
荧光原位杂交(FISH)
膜蛋白质
Protein+Antibody+Mass
小麦胚蛋白
选择您的所在地
Please Select
Australia
Austria
Brazil
Canada
China
Czech Republic
Denmark
Finland
France
Germany
Greece
Hong Kong
India
Ireland
Israel
Italy
Japan
New Zealand
Pakistan
Singapore
South Korea
Spain
Sweden
Switzerland
Taiwan
Thailand
United Kingdom
USA
Vietnam
-------------
Afghanistan
Albania
Algeria
Amer.Virgin Is.
Andorra
Angola
Anguilla
Antigua/Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Belarus
Belgium
Belize
Benin
Bhutan
Bolivia
Bosnia-Herz.
Botswana
Brazil
Brit.Virgin Is.
Brunei Daruss.
Bulgaria
Burkina Faso
Burma
Burundi
Cambodia
Cameroon
Canada
Cape Verde
CAR
Cayman Islands
Chad
Chile
China
Colombia
Costa Rica
Cote d'Ivoire
Croatia
Cuba
Czech Republic
Dem. Rep. Congo
Denmark
Djibouti
Dominica
Dominican Rep.
Dutch Antilles
East Timor
Ecuador
Egypt
El Salvador
Equatorial Guin
Eritrea
Estonia
Ethiopia
Falkland Islnds
Fiji
Finland
France
French Guayana
Gabon
Gambia
Georgia
Germany
Ghana
Greece
Greenland
Grenada
Guam
Guatemala
Guinea
Guinea-Bissau
Guyana
Haiti
Honduras
Hong Kong
Hungary
Iceland
India
Indonesia
Iran
Iraq
Ireland
Israel
Italy
Jamaica
Japan
Jordan
Kazakhstan
Kenya
Kiribati
Kuwait
Kyrgyzstan
Laos
Latvia
Lebanon
Lesotho
Liberia
Libya
Lithuania
Luxembourg
Macau
Macedonia
Malawi
Malaysia
Mali
Mauretania
Mexico
Minor Outl.Isl.
Moldova
Mongolia
Montserrat
Morocco
Mozambique
Nepal
Netherlands
New Zealand
Nicaragua
Niger
Nigeria
Norway
Oman
Pakistan
Palau
Palestine
Panama
Pap. New Guinea
Paraguay
Peru
Philippines
Poland
Portugal
Puerto Rico
Qatar
Rep.of Congo
Reunion
Romania
Russian Fed.
Rwanda
Saudi Arabia
Senegal
Sierra Leone
Singapore
Slovakia
Slovenia
Somalia
South Africa
South Korea
Spain
Sri Lanka
St Kitts&Nevis
St. Lucia
St. Vincent
Sudan
Suriname
Swaziland
Sweden
Switzerland
Syria
Taiwan
Tajikistan
Tanzania
Thailand
Togo
Trinidad,Tobago
Tunisia
Turkey
Turkmenistan
Uganda
Ukraine
United Kingdom
Uruguay
USA
Utd.Arab Emir.
Uzbekistan
Venezuela
Vietnam
West Sahara
Yemen
Zambia
Zimbabwe
Home
>
Support
>
Troubleshooting Tips
操作问题指南
以下操作问题指南是我们在解决同产品相关问题时建议试验的步骤。您在开始操作实验碰到问题无法解决,利用其它解决方法依旧没办法解决您的问题时,可以从这里着手。在尝试这些步骤时,大多用户都能将他们的问题解决。
如果问题解决技巧不能解决您的问题,请填写“
联系我们
”的表格,Abnova的客服人员会对您的要求一一做出解答。
High background/absorbance in negative control
Read All
1.Over incubation with the substrate
Try decreasing the incubation time to prevent signal over-development.
2.Substrate incubation is performed under the light
After adding substrate, put the plate in the dark immediately.
3.Reused plates
Use a new plate.
4.Substrate buffer is old
Use fresh substrate reagent.
5.The antibody concentration may be too high
Decrease the concentration of the antibody.
6.Insufficient blocking time
Extend the blocking time or use more/different blocking solution.
7.Contamination of the negative control
May be contaminated by other samples or reagent. Avoid overflowing among wells. Pipetting carefully.
8.Insufficient amount of washing for the plates
Make sure wells are filled up with wash buffer and ensure the complete removal of the residual solution from each well. Increase the number of washes.
Low absorbance value
Read All
1.Incorrect wavelength
Make sure that correct wavelength is set to measure the signal.
2.Insufficient incubation time for the substrate solutions
Optimize the incubation time until the plate has developed enough for ELISA reader.
3.Insufficient antibody used
Try to use more concentrated antibody. Incubate the antibody for the recommended amount of time
4.Target protein is not expressed in the sample
Search information related to the expression profile of the target protein. Increase the amount of sample used or use more sensitive detection reagents.
Poor duplication or reproducibility
Read All
1.Pipetting error
Be careful upon sample loading and avoid the presence of bubbles in wells.
2.Uneven coating
Check the coating procedure and use an appropriate ELISA plates.
Back to Top
条件
|
隐私声明
|
版权和商标
© 2013 Abnova有限公司 版权所有
If you have a question or comment, please select the topic you would like to contact.
*
Topic
Please Select
Content
Suggestion
Website
Other
*
Email address
*
Name
Mr.
Ms.
Mrs.
Dr.
*
Country
Please Select
Australia
Austria
Canada
China
Czech Republic
Denmark
Finland
France
Germany
Greece
India
Ireland
Israel
Italy
Japan
Singapore
South Korea
Spain
Sweden
Switzerland
United Kingdom
USA
-------------
Afghanistan
Albania
Algeria
Amer.Virgin Is.
Andorra
Angola
Anguilla
Antigua/Barbuda
Argentina
Armenia
Aruba
Australia
Austria
Azerbaijan
Bahamas
Bahrain
Bangladesh
Belarus
Belgium
Belize
Benin
Bhutan
Bolivia
Bosnia-Herz.
Botswana
Brazil
Brit.Virgin Is.
Brunei Daruss.
Bulgaria
Burkina Faso
Burma
Burundi
Cambodia
Cameroon
Canada
Cape Verde
CAR
Chad
Chile
China
Colombia
Costa Rica
Cote d'Ivoire
Croatia
Cuba
Czech Republic
Dem. Rep. Congo
Denmark
Djibouti
Dominica
Dominican Rep.
Dutch Antilles
East Timor
Ecuador
Egypt
El Salvador
Equatorial Guin
Eritrea
Estonia
Ethiopia
Falkland Islnds
Fiji
Finland
France
French Guayana
Gabon
Gambia
Georgia
Germany
Ghana
Greece
Grenada
Guam
Guatemala
Guinea
Guinea-Bissau
Guyana
Haiti
Honduras
Hong Kong
Hungary
Iceland
India
Indonesia
Iran
Iraq
Ireland
Israel
Italy
Jamaica
Japan
Jordan
Kazakhstan
Kenya
Kiribati
Kuwait
Kyrgyzstan
Laos
Latvia
Lebanon
Lesotho
Liberia
Libya
Liechtenstein
Lithuania
Luxembourg
Macau
Macedonia
Malawi
Malaysia
Mali
Malta
Mauretania
Mexico
Moldova
Mongolia
Montserrat
Morocco
Mozambique
Nepal
Netherlands
New Zealand
Nicaragua
Niger
Nigeria
Norway
Oman
Pakistan
Palau
Palestine
Panama
Pap. New Guinea
Paraguay
Peru
Philippines
Poland
Portugal
Puerto Rico
Qatar
Rep.of Congo
Reunion
Romania
Rwanda
Saudi Arabia
Senegal
Serbia/Monten.
Sierra Leone
Singapore
Slovakia
Slovenia
Somalia
South Africa
South Korea
Spain
Sri Lanka
St Kitts&Nevis
St. Lucia
St. Vincent
Sudan
Swaziland
Sweden
Switzerland
Syria
Taiwan
Tajikistan
Tanzania
Thailand
Togo
Trinidad,Tobago
Tunisia
Turkey
Turkmenistan
Uganda
Ukraine
United Kingdom
Uruguay
USA
Utd.Arab Emir.
Uzbekistan
Venezuela
Vietnam
West Sahara
Yemen
Zambia
Zimbabwe
*
Your message
*
Verification code
Your message has been sent successfully!