Stem cells are a class of undifferentiated cells that are able to differentiate into specialized cell types. They are distinguished from other cell types by two important characteristics: (i)potency or (ii)potential to differentiate into different cell types. When a stem cell divides, each new cell has the potential either to remain a stem cell or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell. There are two types of stem cells from human:
Embryonic stem cells (ES cells): They are isolated from the inner cell mass of blastocysts. ES cells are pluripotent and can develop into each of the more than 200 cell types of the adult body when given sufficient and necessary stimulation for a specific cell type.
Adult stem cells: They are found in adult tissues such as the brain, bone marrow, blood, blood vessels, skeletal muscles, skin, and the liver. They remain in a quiescent or non-dividing state for years until activated by disease or tissue injury. Compared to ES cells, adult stem cells are thought to be limited to differentiating into different cell types of their tissue of origin.
For the study of human stem cells, Abnova offers a collection of stem cell culture products including MC-Hydrogel for cultivation and formation of embryoid bodies, Stemaxtrix® for feeder-independent cell proliferation, Mouse Conditioned Media for maintenance of cell growth, and Stem Cell Keeper™ as a vitrification solution for cell preservation. These products together with Abnova’s extensive collection of stem cell related antibodies are ready to accelerate your research!
MC-Hydrogel is a thermo-sensitive, methylcellulose hydrogel for creating a low attachment surface for embryoid body or cell aggregate formation. This thermo-sensitive MC-Hygrogel exists as liquid form at room temperature (25-28°C), and semi-solid form at incubated temperature (37°C). MC-Hydrogel supports and promotes the formation of embryoid bodies while maintaining cell line bioactivity and size.
Stematrix® is a human matrix derived from normal human fibroblast for feeder-independent human embryonic stem cell (hESC), induced plutipotent stem cell (iPSC), and human mesenchymal cell (hMSC) culture from bone marrow and cord blood. Stematrix® can increases the proliferation rate of the hMSC from bone marrow and cord blood while maintaining their undifferentiated status, especially after long-term culture. Stematrix® can be used in conjunction with Mouse Condition Media (Catalog # U0020).
Mouse conditioned media is for human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC) culture. This mouse embryonic fibroblast (MEF) conditioned media is prepared and modified according to the protocol published by Pick, M. et al. (1). Serum-free media [DMEM/F12 supplemented with 20% Knockout(TM) serum replacement, 0.1 mM beta-mercaptoethanol, 1 mM L-glutamine, 1% MEM non-essential amino acids, and 4 ng/mL basic fibroblast growth factor] is conditioned by inactivated-MEF cells at 37°C for 24 hours.
Human embryonic stem cell (hESC) and induced pluripotent stem cell (iPSC) are very sensitive to freezing and thawing damage; their survival rate after cryopreservation by slow cooling is low. Stem Cell Kepper™ is a ready-to-use vitrification solution for hESC and iPSC preservation.
This video shows a novel method of forming human embryoid bodies in a polystyrene dish surface-coated with a temperature-responsive methylcellulose hydrogel. [Reference: Biomacromolecules. 2007 Sep;8(9):2746-52]