FISH (Fluorescence In Situ Hybridization) is a cytogenetic technique used to detect and localize the presence or absence of specific DNA sequences on chromosomes. This technique is based on the mechanism of nucleic acid base pairing, only those parts of the chromosome with high degree of sequence complementarity will be recognized and bound by fluorescent probes. Scientists can find out the position of the fluorescent probe bound to the chromosomes by using fluorescence microscopy. This powerful technique enables researchers to rapidly identify a range of chromosomal aberrations across the genome, including those causing mental retardation, various cancers, birth defects, etc.
Abnova has a range of FISH probes collections for the detection of gene amplification, gene loss, break-apart of a gene, genes fusion and chromosomal aneuploidy. Each FISH probe product has either a single, a pair, or multiple numbers of locus-specific, fluorophore-labeled probe(s) originated from a bacterial artificial chromosome (BAC) library.
Single Color FISH Probes
These products are provided in 5X concentrated format to allow mixing of up to 5 single color FISH probes in a single hybridization assay.
Chromosome FISH Probes: These chromosome specific FISH probes can be used to identify specific chromosomes of interest. Each chromosome FISH probe has five different colors of choice, including FITC, Texas Red, Cy5, DEAC and R6G.
Subtelomere FISH Probes: Subtelomeres have been found to be relative gene-rich. Cryptic deletions and rearrangements in these regions have been associated with mental retardation and congenital abnormalities. FISH probes specific for these regions are able to detect such subtle rearrangements. Each Subtelomere FISH probe has five different colors of choice, including FITC, Texas Red, Cy5, DEAC and R6G.
Dual Color FISH Probes
These products are ready to use, each has a pair of locus-specific, fluorophore-labeled probes with different colors. These products are used to detect gene amplification, gene loss, gene split and gene translocation.
Gene FISH Probes: Gene FISH probes are used for identification of specific gene amplification / gene loss.
Split FISH Probes: Split FISH probes are used for identification of gene split (break-apart of a gene).
Translocation FISH Probes: Translocation FISH probes are used for identification of gene translocation (gene fusion).
Made to Order FISH Probes: These FISH probes are currently under development, most of the products in this category are Gene FISH probes. If customer is interested in these products, Abnova will prioritize to develop and produce them immediately for the customer.
Multiple Color FISH Probes
Prenatal FISH Probes: Aneuploidies of 5 chromosomes (13, 18, 21, X, Y) account for 95% of the chromosomal aberrations that cause infants born with defects. The products in this category each comprise of different combinations of fluorophore-labeled probes specific for chromosomes 13, 18, 21, X, and/or Y.
Accessory Kits & Reagents
FFPE FISH PreTreatment Kit: Pre-treatment is an essential step for effective FISH on Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections. This kit contains key reagents used to pretreat FFPE tissue sections from dewaxing with xylene through proteolytic digestion to help permeabilization of cell membranes to facilitate penetration of fluorescence labeled probes.
Other reagents: These are the collection of miscellaneous reagents that are used in the FISH application, including DAPI Counterstain, FISH Hybridization Buffer, and Antifade Solution.
FISH is a technique used to identify and localize the presence or absence of specific DNA sequences on cells and tissues. You can use FISH probes for the detection of gene amplification, loss and translocation. Each FISH probe product has a pair of locus-specific, fluorophore-labeled probes originated from a bacterial artificial chromosome (BAC) library.