Abnova has extensive experience in generating high quality antisera to a wide variety of antigens (peptides, phospho-peptides, proteins, and cDNAs). Using a low dose immunization technique in combination with a proprietary adjuvant, we are able to generate a high-titer, high-affinity antibody response against the immunogen in mice, rats, and rabbits.
Polyclonal Antibody Development Workflow
Evaluation of Standard Antibody Production Service
To carrier protein KLH/BSA/OVA
(A cysteine is automatically added to the C-terminus or N-terminus of the peptide)
Protein Preparation
2~3
V
V
cDNA sourcing
Cloning and sequencing
Protein expression
Immunization
8 ~ 12
V
V
V
V
V
V
V
V
V
V
5~8 injections per animal (3 mice or 3rats)
Sera collection
Report
1
ELISA data*
V
V
V
V
V
V
V
V
V
V
WB data (mammalian overexpression lysate)
V
V
Purification**
2
By protein A purification Final deliverable: > 0.2 mg purified antibody for mice or > 5 mg purified antibody for rats
V
V
V
V
By affinity purification (for rats only)
Final deliverable: > 0.5 mg purified antibody
V
V
V
V
V
Total Lead time (weeks)
10~12
12~14
12~14
17~20
17~20
17~20
19~20
19~20
12~16
12~16
Total Price (USD/EUR)
$750
$1,100
$1,500
$1,850
$2,150
$2,900
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Rabbit
Service Items
Time
(weeks)
Antigen Supplied by Customer
Antigen Supplied by Abnova
Peptide
Phospho- peptide
Protein
cDNA
Peptide Design & Synthesis
4 ~ 5
Customer-supplied antigen
V
V
V
7~20-mer (Purity > 80%)
V
V
V
Single site phosphorylation (Ser, Tyr, Thr)
V
Conjugation
1
V
V
V
To carrier protein KLH/BSA/OVA
(A cysteine is automatically added to the C-terminus or N-terminus of the peptide)
Protein Preparation
2~3
V
V
cDNA sourcing
Cloning and sequencing
Protein expression
Immunization
10 ~ 12
V
V
V
V
V
V
V
V
V
V
5~8 injections per rabbit (2 rabbits)
Sera collection
Report
1
ELISA data*
V
V
V
V
V
V
V
V
V
V
WB data (mammalian overexpression lysate)
V
V
Purification**
2
By protein A purification Final deliverable: 20-50 mg purified antibody
V
V
V
V
By affinity purification Final deliverable: 1-5 mg purified antibody
V
V
V
V
V
Total Lead time (weeks)
11~14
13~16
13~16
17~20
17~20
17~20
19~20
19~20
12~16
12~16
Total Price (USD/EUR)
$750
$1,300
$1,600
$1,850
$2,150
$2,900
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Note: * Polyclonal antibody sera will be QC utilizing ELISA format for reactivity against the immunogen.
** This is the average yield. Actual yield will vary for each Pab.
The above list price is only used for direct orders made to Abnova.
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