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TK1 monoclonal antibody (M02), clone F12
目录号 # : H00007083-M02
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100 ug
USD $ 319
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Product Description: Mouse monoclonal antibody raised against a full length recombinant TK1.
Immunogen: TK1 (AAH07986.1, 1 a.a. ~ 234 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence: MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN
Quality Control Testing: Antibody Reactive Against Recombinant Protein.
Storage Buffer: In 1x PBS, pH 7.2
Storage Instruction: Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
1.
Early assessment of tumor response to JAC106, an anti-tubulin agent, by 3'-deoxy-3'-[(18)F]fluorothymidine in preclinical tumor models.
2.
The histone deacetylase inhibitor PXD101 increases the efficacy of irinotecan in in vitro and in vivo colon cancer models.
Western Blot (Cell lysate) TK1 monoclonal antibody (M02), clone F12 Western Blot analysis of TK1 expression in Hela NE ( Cat # L013V3 ).
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Western Blot (Cell lysate) TK1 monoclonal antibody (M02), clone F12. Western Blot analysis of TK1 expression in Jurkat ( Cat # L017V1 ).
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Western Blot (Cell lysate) TK1 monoclonal antibody (M02), clone F12. Western Blot analysis of TK1 expression in MCF-7 ( Cat # L046V1 ).
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Western Blot (Transfected lysate) Western Blot analysis of TK1 expression in transfected 293T cell line by TK1 monoclonal antibody (M02), clone F12.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to TK1 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5 ug/ml]
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Immunoprecipitation Immunoprecipitation of TK1 transfected lysate using anti-TK1 monoclonal antibody and Protein A Magnetic Bead (U0007 ), and immunoblotted with TK1 MaxPab rabbit polyclonal antibody.
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Sandwich ELISA (Recombinant protein) Detection limit for recombinant GST tagged TK1 is approximately 0.03ng/ml as a capture antibody.
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RNAi Knockdown (Antibody validated) Western blot analysis of TK1 over-expressed 293 cell line, cotransfected with TK1 Validated Chimera RNAi ( Cat # H00007083-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TK1 monoclonal antibody (M02), clone F12 (Cat # H00007083-M02 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
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Western Blot (Transfected lysate)
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Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
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Sandwich ELISA (Recombinant protein)
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RNAi Knockdown (Antibody validated)
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Gene Description: thymidine kinase 1, soluble
Other Designations: thymidine kinase 1 soluble isoform,thymidine kinase-1
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