Goat polyclonal antibody raised against synthetic peptide of CFH.
Immunogen:
A synthetic peptide corresponding to human CFH.
Sequence:
C-HLVPDRKKDQYK
Host:
Goat
Theoretical MW (kDa):
139
Reactivity:
Human
Specificity:
This antibody is expected to recognize isoform a (NP_000177.2) only.
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
ELISA (1:32000) Western Blot (0.03-0.1 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Publication Reference
1.
Complement factor H polymorphism in age-related macular degeneration. Klein RJ, Zeiss C, Chew EY, Tsai JY, Sackler RS, Haynes C, Henning AK, SanGiovanni JP, Mane SM, Mayne ST, Bracken MB, Ferris FL, Ott J, Barnstable C, Hoh J.Science. 2005 Apr 15;308(5720):385-9. Epub 2005 Mar 10.
Applications
Western Blot (Tissue lysate)
CFH polyclonal antibody (Cat # PAB6830) staining (0.03 ug/mL) of human lung lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short concensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq