Goat polyclonal antibody raised against synthetic peptide of TERF1.
Immunogen:
A synthetic peptide corresponding to human TERF1.
Sequence:
C-TMKKLKLISSDSED
Host:
Goat
Theoretical MW (kDa):
48.2, 50.2
Reactivity:
Human
Specificity:
This antibody is expected to recognize both human isoforms of this protein according to NP_003209.1 and NP_059523.1.
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Quality Control Testing:
Antibody Reactive Against Synthetic Peptide.
Recommend Usage:
ELISA (1:8000) Western blot (1-3 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Publication Reference
1.
A human telomeric protein. Chong L, van Steensel B, Broccoli D, Erdjument-Bromage H, Hanish J, Tempst P, de Lange T.Science. 1995 Dec 8;270(5242):1663-7.
Applications
Western Blot (Cell lysate)
TERF1 polyclonal antibody (Cat # PAB6118) staining (2 ug/mL) of HeLa lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
This gene encodes a telomere specific protein which is a component of the telomere nucleoprotein complex. This protein is present at telomeres throughout the cell cycle and functions as an inhibitor of telomerase, acting in cis to limit the elongation of individual chromosome ends. The protein structure contains a C-terminal Myb motif, a dimerization domain near its N-terminus and an acidic N-terminus. Two transcripts of this gene are alternatively spliced products. [provided by RefSeq
Other Designations:
telomeric repeat binding factor 1,telomeric repeat binding protein 1