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CRK polyclonal antibody
- 目录号 # : PAB3512
- 访问频率 :
- 尺寸
- 价格
- 库存
- 可得性
- 购物车
- 100 ug
- USD $ 319
- order now, ship in 5 days
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Phone : +886-2-87511888
Fax : +886-2-87511166- 产品会直接装运至客户处。
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of CRK.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to residues surrounding S41 of human CRK.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:10-50)
The optimal working dilution should be determined by the end user.
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Avian and 1918 Spanish influenza a virus NS1 proteins bind to Crk/CrkL Src homology 3 domains to activate host cell signaling.
Heikkinen LS, Kazlauskas A, Melen K, Wagner R, Ziegler T, Julkunen I, Saksela K.J Biol Chem. 2008 Feb 29;283(9):5719-27. Epub 2007 Dec 28.
- 2.
- The carboxyl-terminal SH3 domain of the mammalian adaptor CrkII promotes internalization of Listeria monocytogenes through activation of host phosphoinositide 3-kinase.
Dokainish H, Gavicherla B, Shen Y, Ireton K.Cell Microbiol. 2007 Oct;9(10):2497-516.
- 3.
- Signaling adaptor protein Crk is indispensable for malignant feature of glioblastoma cell line KMG4.
Wang L, Tabu K, Kimura T, Tsuda M, Linghu H, Tanino M, Kaneko S, Nishihara H, Tanaka S.Biochem Biophys Res Commun. 2007 Nov 3;362(4):976-81. Epub 2007 Aug 27.
- Applications
- Western Blot (Transfected lysate)
- Western blot analysis of CRK (arrow) using CRK polyclonal antibody (Cat # PAB3512).
293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CRK gene (Lane 2).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CRK polyclonal antibody (Cat # PAB3512) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 1398
- Protein Accession#:
- NP_005197;P46108
- Gene Name:
- CRK
- Gene Alias:
- CRKII
- Gene Description:
- v-crk sarcoma virus CT10 oncogene homolog (avian)
- Omim ID:
- 164762
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described. [provided by RefSeq
- Other Designations:
- avian sarcoma virus CT10 (v-crk) oncogene homolog,v-crk avian sarcoma virus CT10 oncogene homolog,v-crk sarcoma virus CT10 oncogene homolog
- Interactome
