Immunohistochemistry (1:50-1:200) Western Blot (1:250-1:500) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.5 (40% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with RASSF6 polyclonal antibody (Cat # PAB23152) at 1:250-1:500 dilution.
Immunohistochemical staining of human smooth muscle with RASSF6 polyclonal antibody (Cat # PAB23152) shows moderate cytoplasmic positivity in smooth muscle cells at 1:50-1:200 dilution.
RASSF6 is a member of the RAS association (RA) domain family of RAS effectors/tumor suppressors that mediate some of the growth inhibitory properties of RAS (see HRAS; MIM 190020) oncogenes (Eckfeld et al., 2004 [PubMed 15574778]).[supplied by OMIM
Other Designations:
Ras association (RalGDS/AF-6) domain family 6,putative RAS binding protein