Immunohistochemistry (1:1000-1:2500) Western Blot (1:250-1:500) The optimal working dilution should be determined by the end user.
Storage Buffer:
In PBS, pH 7.5 (40% glycerol, 0.02% sodium azide)
Storage Instruction:
Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot
Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with OSBP2 polyclonal antibody (Cat # PAB21420) at 1:250-1:500 dilution.
Immunohistochemical staining of human duodenum with OSBP2 polyclonal antibody (Cat # PAB21420) strong cytoplasmic positivity in glandular cells at 1:1000-1:2500 dilution.
Oxysterols are byproducts of cholesterol that can have cytotoxic effects on many cell types. The membrane-bound protein encoded by this gene contains a pleckstrin homology (PH) domain and an oxysterol-binding region. It binds oxysterols such as 7-ketocholesterol and may inhibit their cytotoxicity. Alternate transcriptional splice variants have been observed but have not been fully characterized. [provided by RefSeq
Other Designations:
OSBP-related protein 4,OTTHUMP00000028525,oxysterol binding protein-like 1,oxysterol binding protein-related protein 4