Goat polyclonal antibody raised against synthetic peptide of ALDH2.
Immunogen:
A synthetic peptide corresponding to internal region of human ALDH2.
Sequence:
C-DETQFKKILGYIN
Host:
Goat
Theoretical MW (kDa):
50
Reactivity:
Human, Mouse, Rat
Form:
Liquid
Purification:
Antigen affinity purification
Concentration:
0.5 mg/mL
Recommend Usage:
ELISA (1:8000) Sandwhich ELISA (1-2 ug/mL) Western Blot (0.03-0.1 ug/mL) The optimal working dilution should be determined by the end user.
Storage Buffer:
In 0.5 mg/mL Tris saline, pH 7.3 (0.02% sodium azide, 0.5% BSA)
Storage Instruction:
Store at -20°C. Aliquot to avoid repeated freezing and thawing.
Note:
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Applications
Western Blot (Tissue lysate)
ALDH2 polyclonal antibody (Cat # PAB18541) (0.03 ug/mL) staining of mouse liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
ELISA
Sandwich ELISA
ALDH2 polyclonal antibody (Cat # PAB18541) (1.5 ug/mL) as the reporter with the capture rabbit antibody (2.5 ug/mL).
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq